In vitro EVALUATION OF HYDROALCOHOLIC EXTRACTS OF MYCELIUM, BASIDIOMATA AND SPENT SUBSTRATE OF Pleurotus ostreatus AGAINST Haemonchus contortus

<p><strong>Background. </strong>The edible mushroom <em>Pleurotus ostreatus</em> is a potential alternative for the control of gastrointestinal nematodes due to their nematoxins and secondary metabolites. <strong>Objective. </strong>To evaluate <em>in vitro</em> anthelmintic activity hydroalcoholic extracts mycelium, basidiomata spent substrate <em>P. against eggs unsheathed larvae <em>Haemonchus contortus</em>. <strong>Methodology. mycelium was extracted with ethanol–water (70:30) methanol–water which were concentrated obtain (MHA), (BHA) extract (SMSE) extracts. The <em>H. contortus</em> exposed different in 96-well plates. tests used egg-hatch larval mortality tests. Three exposure times, 24, 48 72 h, considered. data analyzed factorial design comparison means by Duncan test. <strong>Results. percentage effectiveness (MHA) 93 96.8% at 12.8 240 mg/mL (respectively) after h. Also, observed SMSE BHA eggs, values 87.2 100%, respectively, 25.6 However, showed no larvae, while less than 70% corresponding activity. <strong>Implications.</strong> This an exploratory study tests; vivo</em> studies are still needed. <strong>Conclusion. MHA contortus</em>.</p>